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find Keyword "假丝酵母菌" 5 results
  • 手术治疗假丝酵母菌相关人工心脏瓣膜心内膜炎一例

    Release date:2016-08-30 05:47 Export PDF Favorites Scan
  • 复发性外阴阴道假丝酵母菌病巩固治疗的方法探讨

    目的 观察并评价两种治疗方案对复发性外阴阴道假丝酵母菌病(RVVC)患者进行巩固治疗的疗效。 方法 采用随机对照研究,将2009年2月-2011年1月收治的106例应用克霉唑阴道片(500 mg)强化治疗有效的RVVC患者,随机分为两组进行巩固治疗预防复发。A组(53例):克霉唑阴道片500 mg每周阴道用药1次,连续3个月;B组(53例):克霉唑阴道片500 mg,月经干净后第1、3、5、7 天阴道用药,每月一疗程,连用3个月。巩固治疗结束后随访12个月,观察两组疗效。 结果 A组治愈52例,治愈率98.1%,B组治愈46例,治愈率86.8%,两组之间治愈率比较差异有统计学意义(P<0.05);A组治愈病例52例,12个月的随访中失访3例,复发1例,复发率2.0%,B组治愈病例46例,失访5例,复发9例,复发率21.9%,两组复发率比较差异有统计学意义(P<0.05)。 结论 RVVC巩固治疗方案中,克霉唑阴道片500 mg每周用药1次的连续用药方案对降低RVVC复发的疗效明显优于在月经干净后用药一疗程的治疗方案。

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  • Investigation of the Risk Factors for Nosocomial Urinary Tract Candida Infection and the Nursing Intervention Strategies

    目的 探讨假丝酵母菌引起泌尿道医院感染的特点,提出相应的护理干预对策,为医院泌尿道感染的预防控制提供依据。 方法 2011年6月1日-2013年3月31日,在住院患者中开展泌尿道假丝酵母菌的目标监测,分析感染的现状及相关的危险因素,并于2012年12月1日起,采取针对性的护理干预措施,并评估干预效果。 结果 共发现假丝酵母菌引起的泌尿道感染56例,占总泌尿道感染的40.29%。通过采取护理干预措施,使假丝酵母菌引起的泌尿道感染从3.17例/月降为0.50例/月(P<0.05)。 结论 采取相应有效的护理干预措施,对减少假丝酵母菌泌尿道医院感染,保障医疗安全,具有重要的意义。

    Release date:2016-09-07 02:38 Export PDF Favorites Scan
  • FUNCTION OF INTERCELLULAR ADHESION A, FIBRINOGEN BINDING PROTEIN, AND ACCUMULATION-ASSOCIATED PROTEIN GENES IN FORMATION OF STAPHYLOCOCCUS EPIDERMIDIS-CANDIDA ALBICANS MIXED SPECIES BIOFILMS

    ObjectiveTo explore the function of intercellular adhesion A (icaA), fibrinogen binding protein (fbe), and accumulation-associated protein (aap) genes in formation of Staphylococcus epidermidis-Candida albicans mixed species biofilms. MethodsThe experiment was divided into 3 groups:single culture of Staphylococcus epidermidis ATCC35984 (S. epidermidis group) or Candida albicans ATCC10231 (C. albicans group), and co-culture of two strains (mixed group) to build in vitro biofilm model. Biofilm mass was detected by crystal violet semi-quantitative adherence assay at 2, 4, 6, 8, 12, 24, 48, and 72 hours after incubation. XTT assay was performed to determine the growth kinetics in the same time. Scanning electron microscopy (SEM) was used to observe the ultrastructure of the biofilms after 24 and 72 hours of incubation. The expressions of icaA, fbe, and aap genes were analyzed by real-time fluorescent quantitative PCR. ResultsCrystal violet semi-quantitative adherence assay showed that the biofilms thickened at 12 hours in the S. epidermidis and mixed groups; after co-cultured for 72 hours the thickness of biofilm in mixed group was more than that in the S. epidermidis group, and there was significant difference between 2 groups at the other time (P<0.05) except at 72 hours (P>0.05). In C. albicans group, the biofilm started to grow at 12 hours of cultivation, but the thickness of the biofilm was significantly lower than that in the mixed group in all the time points (P<0.05). XTT assay showed that the overall growth speed in the mixed group was greater than that in the C. albicans group, and it was greater than that in the S. epidermidis group at 48 hours; there was no significant difference in the growth speed between the mixed groups and the S. epidermidis group in the other time points (P>0.05) except at 12 hours (P<0.05). The absorbance (A) value in the mixed group was lower than that in the S. epidermidis group at 2 and 4 hours, but no significant difference was shown (P>0.05); the A value of mixed group was significantly higher than that of the C. albicans group after 6 hours (P<0.05). SEM observation showed that mature biofilms with complex structure formed in all groups. The real-time fluorescent quantitative PCR showed the expressions of fbe, icaA, and aap genes in mixed group increased 1.93, 1.52, and 1.46 times respectively at 72 hours compared with the S. epidermidis group (P<0.05). ConclusionMixed species biofilms have more complex structure and are thicker than single species biofilms of Staphylococcus epidermidis or Candida albicans, which is related to increased expressions of the icaA, fbe, and aap genes of Staphylococcus epidermidis.

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  • In vivo study on the effects of intercellular adhesion operon of Staphylococcus epidermidis on the inflammation associated with bacteria-fungal mixed biofilm

    ObjectiveTo study the effect of intercellular adhesion (ica) operon of Staphylococcus epidermidis on the inflammation associated with mixed biofilm of Staphylococcus epidermidis and Candida albicans on endotracheal tube material in rabbits. MethodsThe standard strains of Staphylococcus epidermidis RP62A (ica operon positive, positive group) and ATCC12228 (ica operon negative, negative group) were taken to prepare a bacterial solution with a concentration of 1×106 CFU/mL, respectively. Then, the two bacterial solutions were mixed with the standard strain of Candida albicans ATCC10231 of the same concentration to prepare a mixed culture solution at a ratio of 1∶1, respectively. The mixed culture solution was incubated with endotracheal tube material for 24 hours. The formation of mixed biofilm on the surface of the material was observed by scanning electron microscope. Thirty New Zealand rabbits, aged 4-6 months, were divided into two groups (n=15), and the endotracheal tube materials of the positive group and the negative group that were incubated for 24 hours were implanted beside the trachea. The body mass of rabbits in the two groups was measured before operation and at 1, 3, and 7 days after operation. At 1, 3, and 7 days after operation, the levels of interleukin 1β (IL-1β), IL-6, tumor necrosis factor α (TNF-α), and monocytechemotactic protein 1 (MCP-1) were detected by using an ELISA test kit. At 7 days after operation, the formation of mixed biofilm on the surface of the endotracheal tube materials was observed by scanning electron microscope, the inflammation and infiltration of tissues around the materials were observed by HE staining, and the bacterial infections in heart, lung, liver, and kidney were observed by plate colony counting method.ResultsScanning electron microscope observation showed that the mixed biofilm structure was obvious in the positive group after 24 hours in vitro incubation, but no mixed biofilm formation was observed in the negative group. In vivo studies showed that there was no significant difference in body mass between the two groups before operation and at 1, 3, and 7 days after operation (P>0.05). Compared with the negative group, the levels of MCP-1 and IL-1β at 1 day, and the levels of IL-1β, MCP-1, IL-6, and TNF-α at 3 and 7 days in the positive group all increased, with significant differences (P<0.05). Scanning electron microscope observation showed that a large amount of Staphylococcus epidermis and mixed biofilm structure were observed in the positive group, and a very small amount of bacteria was observed in the negative group with no mixed biofilm structure. HE staining of surrounding tissue showed inflammatory cell infiltration in both groups, and neutrophils and lymphocytes were more in the positive group than in the negative group. There was no significant difference in the number of bacterial infections in heart and liver between the two groups (P>0.05). The number of bacterial infections in lung and kidney in the positive group was higher than that in negative group (P<0.05).ConclusionIn the mixed infection of Staphylococcus epidermidis and Candida albicans, the ica operon may strengthen the structure of the biofilm and the spread of the biofilm in vivo, leading to increased inflammatory factors, and the bacteria are difficult to remove and persist.

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