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find Author "刘庆淮" 21 results
  • 热休克蛋白90抑制剂治疗新生血管性眼病的研究现状

    热休克蛋白90(HSP90)作为一种广泛存在的分子伴侣,在新生血管性疾病的发生发展中起重要作用。HSP90抑制剂能抑制HSP90活性,引起与细胞增生、细胞周期调节、细胞凋亡有关的重要信号蛋白选择性降解,并通过与磷脂酰肌醇-3激酶/蛋白激酶B和类风湿性关节炎因子/蛋氨酸脑啡肽/促分裂原活化蛋白激酶等信号通路中的多种HSP90客户蛋白分子相互作用,降低血管生成因子的表达,从而抑制血管内皮细胞的增生和存活。研发特异性的HSP90抑制剂药物已经成为治疗肿瘤及相关新生血管性疾病的新选择。其可能成为治疗新生血管性眼病的全新靶点。

    Release date:2016-09-02 05:26 Export PDF Favorites Scan
  • 中心性浆液性脉络膜视网膜病变与相关激素水平变化的研究现状

    中心性浆液性脉络膜视网膜病变(CSC)确切病因和发病机制尚不明确。近年的研究表明,患者体内儿茶酚胺、糖皮质激素、盐皮质激素、性激素等多种激素水平的改变与CSC的发生发展有密切关系。儿茶酚胺可能通过脉络膜血管肾上腺素能受体导致脉络膜微循环损害,糖皮质激素可能影响脉络膜血流和视网膜色素上皮细胞,而性激素可能通过逆转儿茶酚胺和肾上腺皮质激素在CSC中发挥作用。进一步研究CSC与体内相关激素变化的相互关系,可为CSC发病机制和治疗研究提供新的思路。

    Release date:2016-09-02 05:37 Export PDF Favorites Scan
  • 色素上皮衍生因子与视网膜疾病的研究进展

    研究表明色素上皮衍生因子( pigment epithelium-derived factor, PEDF) 能影响视网膜分化、发育和成熟,对视网膜缺血性损伤有促进修复作用,是新生血管的天然抑制剂。现将PEDF的研究历史、PEDF的结构、生理作用以及与视网膜疾病的关系研究现状综述如 下。 (中华眼底病杂志,2003,19:68-70)

    Release date:2016-09-02 06:00 Export PDF Favorites Scan
  • 局限性脉络膜凹陷一例

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  • Expanding the analysis of optical coherence tomography images

    Optical coherence tomography (OCT), as a high-resolution, non-invasive, in-vivo image method has been widely used in retinal field, especially in the examination of fundus diseases. Nowadays, the modality has been gradually popularized in most of the national basic-level hospitals. However, OCT is only employed as a diagnostic tool in most cases, ophthalmologists lack of awareness of further exploring the information behind the raw data. In the era of fast-developing artificial intelligence, on the basis of standardized information management, a more comprehensive OCT database should be established. Further original image processing, lesion analysis, and artificial intelligence development of OCT images will help improve the understanding level of vitreoretinal diseases among clinicians and assist ophthalmologists to make more appropriate clinical decisions.

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  • 玻璃体腔注射抗血管内皮生长因子单克隆抗体bevacizumab治疗视网膜分支静脉阻塞伴黄斑水肿的疗效观察

    Release date:2016-09-02 05:26 Export PDF Favorites Scan
  • Abnormal expression of αA-crystallin in neural retina in type 2 diabetic rats

    Objective To observe the abnormal expression of alpha;A-crystallin protein in neural retina in type 2 diabetic rats via proteomic technique.Methods Twenty-eight male Sprague-Dawley (SD) rats were randomly divided into the normal control and the diabetic experimental groups with 14 rats in each group.A type 2 diabetes rat model (T2DM) was set up in the diabetic experimental group by feeding high fat diet combined with peritoneal injection of low dose streptozotocin (STZ);the successful diabetes model is with the randomlydetected blood glucose of >16.7 mmol/L.The rats in the control group underwent peritoneal injection of equivalent sodium citrate solution and were fed with normal diet.All of the animals were sacrificed by decapitation 56 days after the induction of diabetes.The eyes were enucleated and the neural retina layers were carefully peeled off and preserved.The total neural retinal proteins were extracted from the control and diabetic groups, respectively,and then subjected to two dimensional gel electrophoresis (2-DE).Some different proteins spots were identified by peptide mass fingerprinting (PMF) as well as by tandem mass spectrometric (MS/MS) measurements.Western blot and indirect immunofluorescence (IMF) were used to confirmed that alpha;A-crystallin protein expression was upregulated in diabetic retina.Results An average of (3122plusmn;37) spots in normal retinas and(2702plusmn;21)spots in diabetic group were found by 2-DE image analysis software; about 150 spots in 2-DE gel of diabetic retinae exhibited statistically significant variations (t>2.77,P<0.05).Compared with normal rats' retinae, diabetic ones presented 68 protein spots of up regulation expression and 82 of downregulation expression in 2DE gel.Furthermore,20 of the 150 protein spots were identified by mass spectrometry.The points of 2369 and 1048 in 2-DE gel, showing high expression in diabetic retinal tissues, were identified as alpha;A-crystallin via PMF.Western blot validated that the expression level of alpha;A-crystallin in diabetic neural retina was much higher than that in the control group. Significantly increased expression of alpha;A-crystallin in nuclear retina in diabetic group was also observed by IMF. Fluorescence was mainly seen in the retinal nuclear layer;alpha;A-crystallin aggregation was detected in the perinuclear region of neurons.Conclusion The expression of alpha;A-crystallin increases in neural retina of early T2DM rats.

    Release date:2016-09-02 05:40 Export PDF Favorites Scan
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    Release date:2016-09-02 06:00 Export PDF Favorites Scan
  • The progress of cell-replacement therapy for age-related macular degeneration

    Based on the pathogenic mechanisms of age-related macular degeneration (AMD), tremendous preclinical and clinical trials have demonstrated that cell transplantation which aim to replace impaired retinal pigment epithelium (RPE) with healthy RPE cells is a promising approach to treat AMD. So far, choices of cell sources mainly are autologous RPE, iris pigment epithelium, fetal RPE, human embryonic stem cell-derived RPE and human induced pluripotent stem cell-derived RPE, and some of them are undergoing clinical researches. Grafting manners in cell-based therapies are various including RPE sheet or RPE-choroid complex transplantation, RPE cell suspension injection, and RPE sheet transplantation with scaffolds. This review is limited to cell-based therapies for RPE that damaged first in the progress of AMD and focus on recent advances in cell sources, transplantation methods, preclinical and clinical trials, and the obstacles that must be overcome.

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  • Clustered regularly interspersed short palindromic repeats/Cas9 gene editing and its research progress in ophthalmology

    Clustered regularly interspersed short palindromic repeats/Cas system is a powerful genome-editing tool for efficient and precise genome engineering both in vitro and in vivo, with the advantages of easy, convenient and low cost. This technology makes it possible to simultaneously mutate multiple genes in a single fertilized egg, thus to study the gene expression, genetic interaction and gene function. Even though this method is still in its immature stage and its stability is inconclusive, making precision models of ocular diseases through genome editing may provide a positive effect to explore gene targeted therapy in genetic eye disease.

    Release date:2016-11-25 01:11 Export PDF Favorites Scan
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