Objective To observe the expression of proteins in light-injured retinal pigment epithelial (RPE) cells. Methods ARPE19 cells were exposed to the cool white light at the intensity of (2200plusmn;300) Lx for 6 hours to set up the light injured model. Cellular soluble proteins was extracted and analyzed by means of twodimensional electrophoresis to find out the changes of protein map of lightinjured RPE cells. Results Cellular soluble proteins had (390plusmn;10) spots on the map, in which 11 spots had obvious difference between the light injured group and the normal control group. In the lightinjured cells, the expressio of 8 proteins increased, 1 decreased, and 2 disappeared. Conclusion Twodimensional electrophoresis can find out the difference of expression of proteins in lightinjured and normal RPE cells.
目的探讨腹腔镜胆囊切除术(LC)致胆管损伤的预防措施。方法回顾性总结我院1999 年10 月至2005 年4月期间1 750例LC中,8例胆管损伤患者的损伤情况和特点。结果胆管横断伤4例，胆总管完全夹闭1例，胆总管3/4夹闭1例，电灼伤迟发性肝总管穿孔1例，肝总管撕裂伤1例。结论术中术者只要能遵循一定的手术程序,掌握LC的重要技术原则和技术要点,就能够很好地防止LC中胆管损伤的发生。
目的：分析汶川地震灾后地震伤员眼科专业相关伤情特点以及伴发伤特点，为处理类似大规模灾难事件提供基础资料。方法：收集汶川地震后绵阳地区及周边4个受灾严重的区市县11家大中型医院(含外援医疗队)诊治地震伤员的眼科相关伤情，对其受伤特点和处置预后进行综合分析。结果：11家医院共诊治眼科专业相关患者226例，前四位排序为：结膜下出血、眼附属器损伤、眼眶骨折、远达性视网膜损伤，常见伴发疾病为颅脑外伤、挤压综合症、耳鼻喉外伤。经及时治疗大部分治愈，仅10例患者遗留功能障碍。结论：高烈度地震灾害在现代有其自身特点，建立灾后相关伤情疾病谱对于处理类似突发公共卫生事件中及时高效抢救和安排资源分配具有重要意义。
Objective To systematically evaluate the effectiveness of aloe vera and its products (e.g. dressing for external use, and gel) for both acute (e.g. laceration, surgical incision, and burn) and chronic (e.g. infectious wound, and artery and venous ulceration) wounds. Methods With “aloe” and “wound” as the index words, such databases as Cochrane Library, PubMed, EMbase, CBM, CNKI and VIP were searched from the inception to April, 2012 to collect the randomized controlled trials (RCTs) about aloe vera or its products for treating acute and chronic wounds. Two authors independently screened literature, extracted data and assessed the risks of bias. Then meta-analysis was conducted using RevMan 5.0 software. Results A total of 15 RCTs involving 773 participants were included, and all studies were of low quality. There were 8 RCTs which evaluated aloe vera for treating acute wounds (burn, haemorrhoidectomy, and skin biopsy), and no RCT reported the incidence of infection. Compared with silver sulfadiazine, aloe vera products could increase the proportion of healing wounds for patients with burn (RR=1.28, 95%CI 1.03 to 1.60, P=0.03 ); compared with antibiotic cream, aloe vera products could reduce the average healing time for patients with burn (MD= ?12.90, 95%CI ?13.29 to ?12.51, Plt;0.000 01); compared with conventional therapy, aloe vera gel could increase the proportion of healed patients with acute surgical wounds (RR=16.33, 95%CI 3.46 to 77.15, P=0.000 4), and could increase the proportion of healed patients with pressure sores (RR=1.73, 95%CI 1.21 to 2.49, P=0.003). Conclusion The application of aloe vera topical agents and dressings is positively significant to both acute and chronic wounds. But this conclusion still needs to be supported by high quality trials.
Objective To assess the protective effect of recombinant human erythropoietin (EPO) on human retinal pigment epithelial (RPE) cells injured by light. Methods Cultured human RPE cells were exposed to light for 12 hours, and the culture was stopped 24 hours later. The 3(4,5dimethylthiazole2y1)2,5diphenyl tetrazolium bromide (MTT) cell viability assay and annexin V flunorescein isothiocyanate/propidium iodium labeling and flow cytometry were used to assess the effects of EPO with different concentration on the cellular viability and apoptosis of human RPE cells. The protective effect and mechanism of EPO on RPE cells injured by light was detected by adding AG490. Results EPO, especially with the concentration of 40 IU/ml, obviously increased the cellular viability of RPE cells and apparently decrease the cellular apoptosis induced by light injury. After adding AG490, the effects of EPO on cellular viability and apoptosis were inhibited. Conclusion It is suggested that EPO can protect the human RPE cells from lightinduced injures, and its protective mechanism works after the combination of EPO and its receptor.