Schwanns cells were obtained from the distal end of the sciatic nerve following Wallerian degeneration of SD rats. These cells were cultured with the anteriorhorn neuron of spinal cord of 14dayold SD rat fetus. The two kinds of cells were separated by a slice. Through the microscope, the dendrites and the morphology changes at the 24th, 48th, 72th, and 96 th hour after culture were observed. It was demonstrated that the Schwanns cells played the role of maintaining the survival of neuron and promoting the growth of dendrites. It was said that the Schwanns cells could secrete neurotrophic factor which made the body enlarged and caused the dendrites enlonged to several times of the body.
In experimental study on pigs was designed to evaluate the changes of the vessel density of super thin skin flaps.By means of stratified sampling, isotropic uniform random section, the vessel density of the supor thin skin flap was obeerved under the MIAS-200 image analyzer. The results showed that the remarkable changes of vascular density were found to begin on the 5th day after operation and was first appeared in the distal port of the survived flap,then,it proceeded gradually from the distal proximally.