目的：调查了解成都市建筑工地工作的流动人口工人的生殖健康现状，为项目的干预打下基础。方法：采用定量调查和定性研究相结合的方法获取相关数据和信息。结果和结论：流动人口中的建筑工人的确是一个获取生殖健康知识和服务方面十分脆弱的人群：1)流动人群中的建筑工人们的健康状况不容乐观。他们大都看似健康，但健康知识贫乏、不重视自身生殖健康、寻求健康服务的行为单一且不安全；2)建筑公司和工地基本不允许女工在工期怀孕；除特殊工种外，工人没有体检；一般只给工人买工伤险，但没有医疗保险；3)流动人群中的建筑工人们对性与生殖健康方面的需求是迫切的。但他们求医难，寻求性与生殖健康服务更难。
【摘要】 目的 探讨颅内原发生殖细胞瘤的临床特征。方法 收集华西医院脑外科2000年1月—2009年3月119例颅内原发生殖细胞瘤的临床资料进行回顾性分析。结果 发病率约占同期颅内肿瘤的 1.1%。男〖DK〗∶女=1.64〖DK〗∶1,平均发病年龄15.4岁。临床表现主要为颅内高压症状及视力视野改变,分别为61、56例；肿瘤主要来源于松果体区及鞍区；64例患者行放疗或放疗加化疗,随访45例。55例患者行手术治疗(其中术后行单纯放疗19例,放疗加化疗5例),随访36例。随访时间3~94个月,平均29.7个月。行放疗和放化疗的患者3、5年生存率明显高于单纯行手术治疗的患者。结论 颅内原发生殖细胞瘤多见于儿童及青少年,男性多见,肿瘤大多位于松果体区及鞍区。临床表现位于松果体区者主要为颅内高压症状等,位于鞍区者主要为视力视野改变、尿崩等。放疗联合化疗是主要治疗手段。
Objective To retrospectively analyze the emergency complications of the patients after oocyte retrieval with assisted reproductive technology (ART), and analyze the corresponding strategies. Methods The clinical data of patients after oocyte retrieval with ART between January and December 2016 were retrospectively anayzed. The postoperative emergency complications were observed. Results A total of 5 013 patients were included in the study. The common emergency complications after oocyte retrieval included vaginal bleeding in 137 cases (2.73%) , ovarian hyperstimulation syndrome (OHSS) in 35 (0.69%), hematuria caused by bladder injury in 11 cases (0.21%), pelvic infection in 3 (0.06%), and vagal reflex in 2 (0.04%). OHSS was related to age, the number of basal follicles, the number of oviposaccharides and the estradiol level on the day of human chorionic gonadotropin injection, but not related to the body mass index and the number of days of gonadotropin use; which might be misdiagnosed most likely. Conclusions OHSS is one of the common and severe emergency complications after oocyte retrieval with ART, which should be concerned. Active treatment of complications is helpful to reduce the incidence of emergency complications after oocyte retrieval with ART.
目的 通过检测人睾丸生殖细胞肿瘤中的Skp2蛋白质异常表达,探讨相关意义。 方法 应用S-P免疫组织化学法检测睾丸生殖细胞肿瘤，正常睾丸组织和慢性睾丸炎组织中Skp2的表达。 结果 睾丸生殖细胞肿瘤中Skp2阳性表达率为74.5%，正常睾丸组织中Skp2阳性表达率为20.0%，在慢性睾丸炎组织中Skp2阳性表达率为40.0%，在3种不同睾丸组织中表达差异有统计学意义（P＜0.05）；Skp2表达与不同组织学类型的睾丸生殖细胞肿瘤无相关性（P＞0.05）；随着临床分期的增高，睾丸生殖细胞肿瘤中的Skp2表达增多，差异无统计学意义（P＞0.05）。 结论 在人睾丸生殖细胞肿瘤中的Skp2高表达，提示细胞周期的异常调控在睾丸生殖细胞肿瘤的发生、分化中起着重要的作用。
ObjectiveTo explore the effect of chronic unpredictable mild stress (CUMS) on the reproductive function of mice and provide a suitable animal model for reproduction and stress. MethodsA total of 240 female Kunming mice were feed for 5 days, and then divided randomly into the control group (n=90) and experimental group (n=150). The mice in the experimental group were stressed by 9 chronic mild unpredictable stress factors for 4 weeks and validated by open field test and sucrose consumption test. We administrated pregnant mare serum gonadotropin (PMSG)/human chorionic gonadotropin (HCG) for induction of superovulation and observed the ovarian response and embryo development potential. ResultsAfter 4-week CUMS stimulation, the weight gain, 2% sugar consumption test and open field test were significantly different between the mice in two groups (P>0.05). After PMSG/HCG was administrated, the antra follicles and preovulatory follicles significantly reduced significantly in the experiment group than that in the control group (P<0.05); the number of oocytes, fertilization rate, 2-cell embryos, D4 embryos, blastocysts, high quality embryo rate and D5 bed points were all significantly decreased in the experiment group than those in the control group (P<0.05). ConclusionThe CUMS female Kunming mice model is a kind of emotional stress animal model with low reproductive function, which is effective, operable and repeatable; it could be used for further study on the mechanism of reproductive medicine.
Since the concept of total mesorectal excision (TME) was proposed and carried out in 1982, the postoperative local recurrence rate of rectal cancer has decreased significantly and the long-term survival rate has increased, thus TME has become the gold standard for middle and low rectal cancer surgery. However, the incidence of postoperative urination and sexual dysfunctions caused by pelvic autonomic nerve injury during TME operation remains high, which needs to be investigated and solved. Over the years, through systematic studies of anatomy, histology and physiology, we have confirmed that dissection anterior to Denonvilliers’ fascia for the anterior wall of rectum, and thus partial resection of Denonvilliers’ fascia, were the leading cause of nerve injury during TME operation. On the contrary, dissection posterior to Denonvilliers’ fascia and entire preservation of Denonvilliers’ fascia are feasible and necessary. Moreover, through anatomical study, Wei’s Line, the surgical marker line of Denonvilliers’ fascia is discovered for the first time, and thus innovative TME (iTME) navigated with Wei’s Line is proposed. The multi-center clinical study has confirmed that compared with traditional TME surgery, the incidences of postoperative urination and sexual dysfunctions in iTME group decrease significantly, with comparable oncologic outcomes, suggesting that iTME surgery could be a better choice for male patients with middle and low rectal cancer at specific stages. This study systematically reviews the research process and operation standard of iTME, and summarizes the application status and future prospects of iTME.