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find Keyword "白细胞介素1β" 4 results
  • A Meta-analysis of the Relationship between Interleukin-1beta and Epileptic Seizure

    目的 系统评价白细胞介素1β(IL-1β)与癫痫发作的相关性。 方法 2012年5月-2013年10月检索PubMed数据库(1985年1月-2013年7月)、Medline数据库(1985年1月-2013年7月)、中国知网(1998年1月-2013年7月)和万方数据库(1996年1月-2013年7月),并辅以谷歌学术搜索引擎进行手工检索。由两名研究者按照研究的纳入与排除标准进行病例对照研究的选择,对入选文献的质量参照“非随机对照临床试验质量评价标准与计分表”进行评价和计分,评估纳入研究的方法学质量并对有效的信息进行数据提取。然后采用RevMan 5.0统计软件进行Meta分析,以加权均数差(WMD)为效应指标。 结果 共纳入5个研究,包括82例癫痫发作的患者和471例正常对照。所纳入的研究未描述详细的抽样方法及其检测方法的特异性。Meta分析结果显示:癫痫发作72 h以内的患者与正常对照人群之间血浆IL-1β的浓度差异无统计学意义[WMD=0.04 pg/mL,95%CI(−0.07,0.16)pg/mL,P=0.46]。 结论 IL-1β可能没有直接参与癫痫的发作,而是通过与其他炎性因子之间的相互作用来实现。因此,需要进一步的研究来证明IL-1β在癫痫发作中的作用。

    Release date:2016-08-26 02:09 Export PDF Favorites Scan
  • AECOPD患者血清IL-1β、IL-1Ra与清晨皮质醇水平的关系

    目的 了解慢性阻塞性肺疾病急性加重期( AECOPD) 患者血清IL-1β、IL-1Ra 及IL-1β/ IL-1Ra 与清晨皮质醇水平的关系。方法 38 例AECOPD 患者在接受糖皮质激素治疗之前, 用酶联免疫吸附试验方法检测患者血清IL-1β、IL-1Ra, 用放射免疫方法检测患者清晨空腹血清皮质醇水平, 并用直线相关分析和方差分析来分析其相关性。结果 血清IL-1β、IL-1β/ IL-1Ra 与皮质醇呈直线负相关, IL-1Ra 与血清皮质醇呈直线正相关( P 均lt;0.05) 。结论 在AECOPD 患者中, IL-1β、IL-1Ra 的变化及IL-1β/ IL-1Ra 失衡参与了神经内分泌失调, 是机体内环境紊乱的重要病理生理机制。

    Release date:2016-08-30 11:56 Export PDF Favorites Scan
  • Escherichia coli lipopolysaccharideinduced endophthalmitis and cytokine expression

    Objective To observe the histopathologic features and expression patterns of tumor necrosis factor-alpha; (TNF-alpha;), interleukin-1beta;(IL-1beta;) and Escherichia coli lipopolysaccharide (LPS) in the rat vitreous with LPS inducedendophthalmitis. Methods Wistar rats were randomly divided into saline control group (SC,136 rats),endophthalmitis group (EO, 168 rats)and blank control group (BC,12 rats).EO group received an intravitreal injection of 5 mu;l LPS; SC group received 5 mu;l sterile saline and no intervention for BC group.Six,12,24,48, and 72 hours,5 and 7 days after injection, intraocular inflammation were observed and the eyes and vitreous were collected for histopathological examination and measurement of TNF-alpha;, IL-1beta; and LPS expression. Results Severe inflammatory responses in the eyes were observed in EO group between six and 72 hours after LPS injection,ocular inflammation subsided seven days after LPS injection. In the vitreous, a peak neutrophil count was observed at 24 hours (1224.64plusmn;132.2) cells/eye that rapidly declined at 72 hours (342.25plusmn;47.7) cells/eye. The levels of TNF-alpha; and IL-1beta; in EO group were peaked at 24 hours with (996.18plusmn;89.45) and(5556plusmn;1440)pg/ L, respectively;Persisted at 48 hours and began to decline rapidly thereafter. Seven days after LPS injection, levels of TNF-alpha; and IL-1beta; returned to baseline with (22.16plusmn;5.84)and (73.7plusmn;18.7) pg/L, respectively. LPS concentration in EO group decrease rapidly at 72 hours with (11.03plusmn;3.41) ng and disappear on days 7 with (0.22plusmn;0.08) ng after LPS injection.Conclusions Massive neutrophils infiltration, high levels expression of TNF-alpha; and IL-1beta; and spontaneous elimination of bacterial elements in vitreous cavity were major pathologic characteristics in this experimental model. The expression patterns of TNF-alpha;,IL-1beta; were in accord with LPS clearance process.

    Release date:2016-09-02 05:41 Export PDF Favorites Scan
  • Inhibitory effects of pigment epithelium derived factor on oxygen-induced retinal neovascularization

    ObjectiveTo study the inhibitory effects of pigment epithelium derived factor (PEDF) on oxygen-induced retinal neovascularization in mice, and to investigate the possible involvement of interleukin-1β (IL-1β) in the neovascular-inhibitory function of PEDF. Methods A total of 140 postnatal day (P)7 C57BL/6 mice were randomly divided into normal control group, oxygen-induced retinopathy (OIR) model group, PEDF treatment group and PBS treatment control group. All mice except normal control group with their mothers were exposed to (75±2)% oxygen environment for 5 days and then kept in room air for another 5 days to establish the OIR model. Mice in normal control group were kept in room air only. At P12 and P14, respectively, mice in PEDF treatment group received intravitreous injections of 1 μl PEDF (2 μg/μl), while PBS treatment control group received the same volume of PBS (10 mmol/L, pH7.4).All mice were euthanized at P17 and eyes were isolated. The changes of retinal vessels were observed on retinal flat mounts and cryosections by fluorescence microscopy. Retinal specimens were prepared for IL-1β protein and mRNA analysis by Western blot and real time fluorescence quantitative reverse transcription-polymerase chain reaction (Real-time RT-PCR). ResultsChanges of retinal vessels had been viewed by fluorescence microscopy on flat-mounted retina, the relative retinal neovascularization areas were significantly increased in OIR model group compared with normal control group (t=15.02, P < 0.01), and the relative retinal neovascularization areas were obviously smaller in PEDF treatment group than those in PBS treatment control group (t=5.96, P < 0.01). Fluorescence staining revealed that retinal vascular tufts were extending from outer plexiform layer (OPL) to ganglion cell layer (GCL) of the retina along with multiple interconnections; Neovascular tufts in OIR model group and PBS treatment control group were presenting distinctly more than those of normal control group and PEDF treatment group. The specific expression levels of IL-1β protein in retinas of OIR mice by Western-blot analysis were higher than those of normal control group(t=3.35, P < 0.05), While these of PEDF treatment group showed a considerable decline in comparison with PBS treatment control group (P < 0.01), and there were no difference in normal control group and PEDF-treated group (F=11.764, P > 0.05). Similarly, expression levels of IL-1β mRNA tested by Real-time RT-PCR were obviously increased in the OIR model group when compared to normal control group(t=4.43, P < 0.01). After treated with PEDF, expression levels of IL-1β mRNA showed a considerable decrease when compared to PBS treatment control group (P < 0.01), and there were no difference in normal control group and PEDF-treated group (F=11.15, P > 0.05). ConclusionsPEDF can inhibit oxygen-induced retinal neovascularization. The mechanism may be related to that PEDF can downregulate the expression of IL-1β in retina.

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