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find Author "连锋" 19 results
  • Effect of Secreted Frizzled-related Protein 2 and Wnt Signaling Pathway in Myocardial Regeneration Treatment

    Abstract: Stem cell paracrine has been considered as the main mechanism to promote infarcted myocardium regeneration and repair of damaged cardiomyocytes. With further research, secreted frizzled-related protein 2 (Sfrp 2) and stem cell paracrine are closely linked to each other. Sfrp 2 can competitively bind to the specific receptor Fz in Wnt signaling pathway, inhibit Wnt signaling pathway to regulates apoptosis, differentiation, and other life processes of stem cells, and therefore becomes a research hotspot in recent years. This review focuses on the mechanism of Sfrp 2/Wnt signal way in stem cell therapy for myocardial infarction.

    Release date:2016-08-30 05:50 Export PDF Favorites Scan
  • 自身成体干细胞移植治疗心肌梗死

    心血管疾病,尤其是心肌梗死已成为人类死亡的主要疾病.自身成体干细胞移植为心肌梗死的治疗带来了新的希望.目前可供移植的种子细胞包括骨骼肌卫星细胞,骨髓干细胞,内皮祖细胞,自体移植避免了免疫排斥和伦理道德问题.近期实验表明移植的干细胞能在心肌疤痕中存活,增强心功能.当前需要进一步研究干细胞移植后与心肌微环境的联系,理想的移植细胞数目和移植技术以及对整个心功能可能产生的影响.

    Release date:2016-08-30 06:32 Export PDF Favorites Scan
  • MicroRNA-129 Promotes Cardiomyogenesis in Bone Marrow Mesenchymal Stem Cells

    Objective To explore the induction of cardiomyogenesis of microRNA-129 (mir-129) in rat bone marrowmesenchymal stem cells (BM-MSCs) and its mechanism. Methods BM-MSCs were isolated from Sprague-Dawley rats and cultured in vitro. Overexpression of mir-129 or both mir-129 and glycogen synthase kinase-3β (GSK-3β) in BM-MSCs was produced with a lentiviral vector system. All the BM-MSCs were divided into four groups: control group (MSCs),Lentiviral vectors+MSCs group (Lv-MSCs),mir-129 transfection group (mir-129-MSCs),and mir-129+GSK-3βdouble transfection group (mir-129+GSK-3β-MSCs). Five-Azacytidine (5-Aza) (10 μmol/L) was used to induce BM-MSCsdifferentiation into cardiomyocytes. On the 1st,5 th,10 th,15 th and 20 th day after induction,realtime-PCR was performedto detect mRNA levels of GATA-4,Nkx2.5 and MEF-2C. On the 10 th,15 th and 20 th day after induction,Western blottingwas performed to examine expression levels of cTnI,Desmin,GSK-3β,phosphorylated β-catenin and dephosphorylated β-catenin. Results Compared with the control group,at respective time points,mRNA levels of cardiomyogenic genes and expression levels of cardiomyocyte-related proteins of mir-129 transfection group were significantly elevated,theexpression level of GSK-3β was significantly decreased,and the ratio of dephosphorylated/phosphorylated β-catenin was significantly elevated. When both mir-129 and GSK-3β were overexpressed in BM-MSCs,mRNA levels of cardiomyogenicgenes and expression levels of cardiomyocyte-related proteins were significantly lower than those of mir-129 transfection group,and the ratio of dephosphorylated/phosphorylated β-catenin was significantly decreased. Conclusion Overexpression of mir-129 can promote cardiomyogenesis of rat BM-MSCs possibly via inhibiting GSK-3β production and thus decreasing the inhibition of phosphorylation of β-catenin which then enters the nucleus and activates downstream signaling pathways that regulate cardiomyogenic differentiation of BM-MSCs.

    Release date:2016-08-30 05:47 Export PDF Favorites Scan
  • 感染性心内膜炎的外科治疗

    摘要: 目的 总结自身瓣膜感染性心内膜炎的外科治疗经验。 方法 2000年1月至2008年6月上海交通大学医学院附属仁济医院收治感染性心内膜炎患者49例,男27例,女22例;年龄14~74岁,平均年龄44.2岁。基础疾病:先天性心脏病12例,包括室间隔缺损、右心室流出道狭窄、主动脉窦瘤和动脉导管未闭等;后天性心瓣膜病34例,包括风湿性二尖瓣病变、主动脉瓣病变和老年性心瓣膜退行性变等。不合并基础心脏疾病3例。所有患者均在低温体外循环下施行先天性心脏病矫治术和心瓣膜置换术;术中尽可能清除赘生物,对受侵犯的组织采用碘伏溶液多次擦洗,再用生理盐水彻底冲洗。术后给予抗生素治疗4~6周。 结果 围术期死亡2例(4.08%),1例因心力衰竭死亡,另1例因严重肺部感染死亡。术后2例患者持续发热,继续给予抗生素治疗2周后,体温渐趋平稳。随访35例(71.43%),随访时间4个月至6年,失访12例。1例室间隔缺损、三尖瓣赘生物患者手术后1个月再次发热,再次入院接受抗生素治疗后治愈。3例发生抗凝治疗并发症,出现牙龈或结膜出血、皮肤瘀斑、血尿、消化道出血,暂停抗凝治疗后好转。随访期间未见感染性心内膜炎复发。 结论 早期诊断、正确把握手术时机,药物与手术相结合是成功治疗感染性心内膜炎的关键。

    Release date:2016-08-30 06:03 Export PDF Favorites Scan
  • The Short and Medium Term Effect on Myocardial Contractile Force after Implantation of Autologous Endothelial Progenitor Cells

    Objective To study the short and medium term effect of myocardial contractile force by implantation of endothelial progenitor cells (EPCs) in the myocardial infarction model. Methods Hundred and twenty SD rats were equally and randomly divided into experimental group and control group (60 rats in each group). Acute myocardial infarction model was created by ligation of LAD. Autologous EPCs were purified from peripheral blood then implanted into the acute myocardial infarct site via topical injection. IMDM were used in control group. Specimens and muscle strip were harvested at 3, 6 weeks, 6, 8 and 12 months after EPCs implantation for contractile force study and to detect the expression of vascular endothelial growth factor(VEGF), basic fibroblast growth factor (bFGF) and Ⅷ factor by immunohistology and video image digital analysis system. Results The expression of VEGF, bFGF and the microvessel counts in experimental group were much higher than those of control group(P〈 0.01) at 3, 6 weeks and 6 months after transplantation. The contractile force in experimental group was better than that in control group(P〈0.01) at the same time. But from 8 months after implantation, the contractile force and so on were not up in the experimental group. Conclusion EPCs, after being implanted into infarct myocardium, shows the ability of improvement of the contractile performance in infarcted myocardium by means of angiogenesis and vasculogenesis and the medium term results are persistent.

    Release date:2016-08-30 06:23 Export PDF Favorites Scan
  • The influence of autologous bone mesenchymal stem cells on myocardial structure and cardiac function after being implantated into acute infarcted myocardium

    Objective To study the influence of autologous bone mesenchymal stem cells (BMSCs) on myocardial structure and cardiac function after being implantated into acute infarcted myocardial site. Methods Bone marrow was aspirated from the posterosuperior iliac spine of Guizhou Xiang swine. After being isolated, cultured and co cultured with 5 azacytidine, either autologous BMSCs (total cells 2×10 6, experimental group, n =12), or a comparable volume of culture medium (control group, n =12), was injected into the left anterior descending(LAD) branch of coronary artery just distal to the ligation site of the LAD. The same volume of BMSCs or culture medium was injected into several spots in the infarcted myocardium. Echocardiographic measurements were performed three or six weeks after implantation to assess the myocardial structure and cardiac function. Results Left ventricular function, including eject fraction(EF), fractional shortening and wall thickening, were higher in experimental group when compared with control group. The thickness of the ventricular wall and septum was also found increased while the left ventricular chamber size was smaller in experimental group. Conclusion Implantation of BMSCs into the infarcted myocardium is believed to attenuate the remodeling process, inhibit the extent of wall thinning and dilatation of the ventricular chamber. BMSCs implantation may also improve the contractile ability of the myocardium and cardiac function.

    Release date:2016-08-30 06:27 Export PDF Favorites Scan
  • Angiogenesis and cytokine secretion induced by autologous bone marrow mesenchymal stem cells implantation into infarcted myocardium

    Objective To study the effect of bone marrow mesenchymal stem cells(MSCs) implantation into infarcted myocardium on cytokine secretion and angiogenesis. Methods 24 Guizhou xiang porcine were equally divided into experimental group and control group randomly. Three ml bone marrow was extracted from the posterior superior iliac spine. MSCs were cultured according to the methods of Wakitani’s. After being co-cultured with 5-azacytidine for 24 hours, these cells were labeled with bromodeoxyuridine(BrdU). Autologus MSCs were implanted into the acute myocardial infarct site both via the distal segment of the ligated left anterior descending artery (LAD) and topical injection. 3 amp; 6 weeks after transplantation, the samples from experimental group and control group were collected to detect the expression of vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF) and Ⅷ factor by immunohistology and video image digital analysis system. Results The expression of VEGF, bFGF and the microvessel counts in the experimental group were much higher than those of control group (Plt;0.01) at 3 and 6 weeks after transplantation. Conclusion MSCs, after being implanted into infarcted myocardium, shows the ability of secreting VEGF, bFGF, with subsequent angiogenesis.

    Release date:2016-08-30 06:27 Export PDF Favorites Scan
  • bFGF缓释微胶囊埋藏部位对兔血管新生的影响

    目的 通过局部心外膜下应用碱性成纤维细胞生长因子(bFGF)缓释微胶囊,探讨其治疗心肌梗死的最佳埋藏部位,为进一步临床应用提供理论依据. 方法 24只新西兰大白兔随机分为对照组(Ⅰ组),空白胶囊组(Ⅱ组),bFGF缓释微胶囊组(Ⅲ组,每只胶囊含bFGF 1μg),每组8只.开胸结扎冠状动脉前降支根部,Ⅱ组、Ⅲ组于左旋支,前降支交界区心外膜下埋藏空白微胶囊,bFGF缓释微胶囊各5只.术后5周,免疫组织化学测定心肌梗死边缘区,胶囊埋藏区微血管数. 结果 与Ⅰ组、Ⅱ组相比较,Ⅲ组心肌梗死边缘区微血管数目明显增多(Plt;0.01),胶囊埋藏区3组间微血管数差别无显著性意义(Pgt;0.05). 结论 bFGF缓释微胶囊组织相容性较好,在靠近缺血心肌的正常心肌心外膜下埋藏,可以达到理想的诱导缺血心肌血管新生的效果.

    Release date:2016-08-30 06:30 Export PDF Favorites Scan
  • miR-190a-5p targeting the C/EBPα-PU.1 pathway promotes the polarization of bone-marrow-derived macrophages from M1-type to M2-type

    ObjectiveTo investigate the effect of miR-190a-5p on the polarization of bone-marrow-derived macrophage (BMDM) induced by lipopolysaccharides to M1- and M2-types.MethodsBMDM (M1-type) induced by bacterial lipopolysaccharide was a M1 group. The macrophage M1-type interfered with negative control miRNA mimics was a NC group. miR-190a-5p mimics interfered with the M1-type of macrophages in the miR-190a-5p group. Morphological changes of macrophages were observed under a microscope, and the proportion of M2-type macrophages (CD206+, F4/80) was detected by flow cytometry. The mRNA expression levels of argininase-1 (Arg1), inducible nitric oxide synthase (iNOS), tumor necrosis factor-α (TNF-α), target gene C/EBPα and PU.1 were detected by fluorescence quantitative PCR to verify whether C/EBPα and PU.1 were potential target genes of miR-190a-5p. The expression of pathway proteins C/EBPα and PU.1 were detected by Western blotting.ResultsAfter miR-190a-5p mimics interfered with macrophage M1-type, the antenna of macrophages elongated and showed long cord M2-type cell morphological characteristics. miR-190a-5p mimics interfered with M1-type macrophages for 24 h, and the percentage of M2-type macrophages increased significantly (P<0.05). Effects of miR-190a-5p simulator on mRNA expression levels of M1-type macrophages included: the expression of iNOS and TNF-α was significantly decreased (P<0.05), the expression of Arg1 marked by M2 macrophages was significantly increased (P<0.05), and the mRNA expression levels of target genes C/EBPα and PU.1 were significantly decreased (P<0.05). Western blotting results showed that the overexpression of miR-190a-5p significantly inhibited the protein expressions of C/EBPα and PU.1, while the miR-190a-5p inhibitor increased the expressions of both proteins.ConclusionmiR-190a-5p can promote the polarization of BMDM from M1-type to M2-type.

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  • Surgical Treatment of Left Ventricular Aneurysm

    ObjectiveTo evaluate the effects of modified left ventricular reconstruction (LVR) and linear repair (LR) to post-infarct left ventricular aneurysm (LVA) and summarize the surgical experience of LVA. MethodsFrom May 2004 to December 2011, 47 patients were admitted in the Department of Cardiovascular Surgery, Renji Hospital Affiliated to Medical College of Shanghai Jiaotong University. There were 25 patients underwent LVR (group LVR, including 21 males and 4 females), 18 patients underwent LR (group LR, including 14 males and 4 females) and 4 patients underwent directly sutured (including 3 males and 1 female). Among them, 42 patients underwent coronary artery bypass grafting (CABG). During 6-24 months'follow-up, left ventricular ejection fraction (LVEF), quality of life and activity were measured. ResultsPostoperative LVEF was significantly higher than preoperative LVEF in group LVR(49.2%±13.6% vs. 32.5%±12.9%, P < 0.05) and group LR (47.5%±11.6% vs. 36.9%±11.6%, P < 0.05). One patient died in LR group (5.5%) and 1 died in LVR group (4.0%), no death occurred in directly sutured surgery. Total mortality was 4.2%. ConclusionLVR and LR are both effective treatment for LVA. Personalized treatment can receive satisfactory short-and long-term outcomes.

    Release date:2016-10-02 04:56 Export PDF Favorites Scan
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