Objective To investigate the application and advancement of hepatocyte t ransplantation ( HCT) .Methods Literatures about the advancement of HCT were reviewed and analyzed. Results There have been manynovel technologies and advancement s in the application of HCT. For example , gene modified cell can be used as seedcell , subcutaneous t ransplantation can be taken when combined with giant molecule material and the encap sulationpreconditioning technique can also carried before operation to improve the rate of survival. Conclusion With moreand deeper understanding of hepatocyte t ransplantation and the development of advanced techniques such as the application of giant molecule , HCT will be extensively used in the clinical t reatment of acute and chronic hepatic diseases.
目的 探讨腹腔镜下乙状结肠癌根治术保留盆腔自主神经的可行性及对术后泌尿、生殖功能的影响。 方法 选取2007年8月－2009年7月60例行腹腔镜下乙状结肠癌根治术患者，手术按标准腹腔镜下乙状结肠癌前切除手术方式操作。术后采用问卷调查方式对患者术后泌尿功能、男性勃起功能、女性性功能进行效果评价。生殖泌尿功能评估包含国际前列腺增生评分（IPSS）、国际勃起功能评分量表（IIEF）、女性性功能评估量表（FSFI）。 结果 58例患者成功保留自主神经，56例接受术后泌尿、生殖功能测定，置放尿管时间为1～7 d，平均3 d，拔除尿管后排尿功能良好（IPSS 0～7分）57例（95.0%），一般（IPSS 8～14分）2例（3.3%），差（IPSS 15～35分）1例（1.7%），术前、术后IPSS评分差异无统计学意义（P=0.075）。30例男性患者术后，射精良好27例（90.0%），一般2例（6.7%），差1例（3.3%）；但是勃起功能测定良好（IIEF 60～75分）28例（93.4%），一般（IIEF 44～59分）1例（3.3%），差（IIEF 5～43分）1例（3.3%），术前、术后IIEF差异无统计学意义。26例女性患者术后性功能评分，良好（FSFI 76～95分） 23例（88.5%），一般（FSFI 58～75分） 2例（7.7%），差（FSFI 4～57分）1例（3.8%）。盆腔神经保留成功的患者术前、术后差异无统计学意义（P=0.122）。 结论 多数腹腔镜下乙状结肠癌根治术患者能有效保留盆腔自主神经，且术后能保留泌尿、生殖功能。
【 Abstract 】 Objective To study the mRNA expression of BC047440 gene in multiplicate malignant tumor tissues and the corresponding adjacent tissues, and to investigate its roles in the carcinogenesis and development of malignant tumors. Methods Forty-eight cases of malignant tumor tissues and their adjacent non-cancerous tissues were examined. The mRNA expression of BC047440 gene in those tissues of liver cancer, cholangiocarcinoma, gastric cancer, carcinoma of large intestine, glioma, and breast cancer were measured by semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR). Results ① The mRNA expressions of BC047440 gene in liver cancer, gastric cancer, cholangiocarcinoma and carcinoma of large intestine were significantly higher than those in their adjacent non-cancerous tissues (Plt;0.05 or 0.01). BC047440 gene were highly expressed in both glioma and its adjacent tissues (Pgt;0.05), and poorly expressed in both breast cancer and its adjacent tissues (Pgt;0.05). ② There were close relationships between BC047440 gene expression and clinicopathologic findings of liver cancer, including tumor size and portal vein invasion (Plt;0.05). ③ There were also close relationships between BC047440 gene expression and different clinical stages in alimentary canal cancers (Plt;0.05). Conclusion The over expression of BC047440 gene may be related with the growth, infiltration and metastasis of some malignant tumors, including liver cancer, cholangiocarcinoma, gastric cancer, carcinoma of large intestine and glioma.