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find Keyword "T淋巴细胞亚群" 10 results
  • Comparison of the Change of T-Lymphocyte Subsets and Immunoglobulin in Postoperative Gastric Carcinoma Patients with Regional Intra-Artery Implantation Pump Chemotherapy Versus Peripheral Venous Chemotherapy

    Objective To observe the turbulence of regional intra-artery implantation chemotherapy and peripheral venous chemotherapy on immunologic function of patients. Methods Two weeks after radical operation of gastric carcinoma, chemotherapy was performed. Eighty-three patients were divided into two groups, one (42 patients) received peripheral venous chemotherapy (PVC) and the other (41 patients) received regional intra-artery implantation pump chemotherapy (RAIPC). The serum T-lymphocyte subsets and immunoglobulin level before and 1-4 days after the chemotherapy were measured. Results After PVC, proportion of CD3 and CD4, CD4/CD8 and IgG, IgA, IgM concentration in PVC group were significantly decreased compared with those before PVC (P<0.05, P<0.01), and it is the same when compared with postRAIPC patients except for CD4 and IgM (P<0.05). In RAIPC group, there were no significant changes in proportion of CD3, CD4 and CD8, CD4/CD8 and IgG, IgA concentration between pre- and post-RAIPC patients. Conclusion After radical operation of gastric carcinoma, RAIPC affects the immunologic function more moderate than PVC.

    Release date:2016-08-28 04:43 Export PDF Favorites Scan
  • EFFECT OF INDUCING DIFFERENATION OF ALL TRANS RETINOIC ACID ON IMMUNITY OF PATIENTS WITH GASTRIC CARCINOMA

    Objective To evaluate the effect of inducing differenation of all trans retinoic acid (ATRA) on immunologic function of patients with gastric cancer. Methods T-lymphocyte subsets(T-Ls) and interleukin-2 receptor(sIL-2R) of 56 patients with gastric cancer after treatment of ATRA were studied. Results In radical gastric cancer resection group, the serum CD3,CD4 and CD4/CD8 rate were higher and sIL-2R were lower than those in the control group, after treatment of ATRA, CD3,CD4 and CD4/CD8 rate and sIL2R were as high as those in the control group. In the non-operative or palliative gastric resection group, CD3,CD4 and CD4/CD8 rates were increased markedly and the serum sIL-2R was decreased significantly than those in the control group. Conclusion ATRA inducing differenation can improve the immunity of the patients with gastric cancer.

    Release date:2016-08-28 05:30 Export PDF Favorites Scan
  • EFFECTS OF TUMOR INFILTRATING LYMPHOCYTES ON CELLULAR IMMUNOLOGIC FUNCTION OF PATIENTS WITH BREAST CANCER

    【Abstract】Objective The effects of tumor infiltrating lymphocytes (TIL) on cellular immunologic function of patients with breast cancer were studied. Methods Twenty five patients with breast cancer were treated by the TIL that were isolated from tissue of tumor. T cell subgroups and natural killer cell (NK cell) activity of peripheral blood, the levels of serum soluble interleukin-2 receptor (sIL-2R) were assayed before and after treatment. Results CD3, CD4, CD4/CD8 and NK cell activity were ascended obviously, and CD8, sIL-2R were descended obviously after the treatment of TIL. Conclusion TIL can enhance the cellular immunologic function of patients with breast cancer.

    Release date:2016-08-28 05:30 Export PDF Favorites Scan
  • EFFECT OF INTRAPERITONEAL HYPERTHERMO-CHEMOTHERAPY ON T-LYMPHOCYTE SUBSETS AND sIL2R LEVEL IN PATIENTS WITH GASTROINTESTINAL MALIGNANCY TUMOR

    To evaluate the effect of intraperitoneal hyperthermo-chemotherapy (IPHC) on immunologic function of patients with gastrointestinal malignant tumor (GMT). The authors determined the serum T-lymphocyte subsets (T-LS) and interleukin-2 receptor (sIL-2R) of 32 patients with GMT after IPHC, and compared the two indexes with 20 healthy control group. Results: Before IPHC the serum CD+3, CD+4, CD+8 and CD+4/CD+8 were higher and sIL-2R were lower than control group, after IPHC, CD+3, CD+4, CD+8 and CD+4/CD+8 increased obviously (P<0.01) and the serum sIL-2R decreased significantly (P<0.01). Conclusion: The IPHC can improve the patients immunologic function.

    Release date:2016-08-29 09:18 Export PDF Favorites Scan
  • EFFECT OF RECOMBINANT HUMAN GROWTH HORMONE ON IMMUNOLOGIC FUNCTION IN PATIENTS WITH GASTROINTESTINAL MALIGNANT TUMOR

    To evaluate effect of recombinant human growth hormone (rhGH) on immunologic function in patients with gastrointestinal malignant tumor (GIMT). Before and 3 weeks after surgical treatment and administration of rhGH, the amount of T lymphocyte subset (T-LS) and soluble interleukin 2 receptor (sIL-2R) level were measured in 12 patients with GIMT, which were compared with 20 cases of normal control and 18 cases of GIMT treated by surgery alone. Result: ①In all GIMT patients, the serum CD+3, CD+4 level and the ratio of CD+4/CD+8 were lower than normal control and the sIL2R level was much higher; ②After operation, the serum CD+3, CD+4 level and the ratio of CD+4/CD+8 of all patients increased, the serum sIL2R level decreased; ③In patients recieved rhGH, the serum CD+3, CD+4 level and the ratio of CD+4/CD+8 were much more increased and the serum sIL-2R level much more decreased than those of surgery alone group. Conclusion: rhGH can enhance the immunologic function of patients with GIMT.

    Release date:2016-08-29 09:20 Export PDF Favorites Scan
  • DETECTATION OF CD3,CD4,CD8 AND CD44 CONTENT OF FINE NEEDLE ASPIRATION SPECIMENTS IN THYROID CANCER PATIENTS

    To search for the relationship between immune state and tumor metastases, CD3,CD4,CD8 and CD44 contents in 13 speciments of fine needle aspiration (FNA) from thyroid cancer patients were detected by the flowcytometry (FCM) and comparison between thyroid cancer and benign tumor was made. The result showed :in thyroid cancer group, CD+3,CD+4 cells and the ratio of CD+4/CD+8 reduced significantly (P<0.01),and CD+8 cell increased significantly (P<0.01), in metastases group,this change was much significantly. CD44 expressed significantly higher in cancer group than that of the benign thyroid neoplasms, and the change was related to the tumor metastases. The results indicate that CD44 can be as a marker of tumor and indicator of metastases. The disturbance of immune system results in active expression of CD44 by tumor cells, so, lead to metastases. It is helpful to the diagnosis of thyroid cancer, assessment of metastases and management in surgery.

    Release date:2016-08-29 03:18 Export PDF Favorites Scan
  • Impact of Recombinant Human Growth Hormone on T Lymphocyte Subsets in Perioperative Patients with Rheumatic Heart Disease: A Randomized Controlled Trial

    Objective To explore the impact of recombinant human growth hormone (rhGH) on T lymphocyte subsets in patients with rheumatic heart disease during the perioperative period of heart valve replacement. Methods A total of 65 patients with rheumatic valvular heart disease who received heart valve replacement in Department of Cardiothoracic Surgery of Xiangyang Central Hospital from June 1, 2011 to March 31, 2012 were enrolled in this double-blind randomized controlled clinical study. All the patients were divided into 2 groups by random number produced by SAS software:the trial group and the control group. There were 35 patients in the trial group including 19 males and 16 females with their average age of 50.57 years, and 30 patients in the control group including 16 males and 14 females with their average age of 49.87 years. Apart from routine cardiac glycosides, diuretics, glucose-insulin-potassium solution, and postoperative anti-infective therapy, patients in the trial group also received subcutaneously injection of rhGH 5 U (1 ml)daily from 1 day before surgery to 3 days after surgery, and patients in the control group received subcutaneously injection of normal saline 1 ml as placebo. Peripheral venous blood samples were taken in the morning 2 days before surgery and 1 st, 3 rd, 7 th day after surgery respectively. Percentages of CD3+, CD4+, CD8+ were examined timely by flow cytometry and CD4+ /CD8+ ratio was calculated. Results In the control group, percentages of CD3+, CD4+ and CD4+ /CD8+ ratio on the 1st, 3rd, 7th postoperative day were significantly lower than preoperative levels, and percentages of CD8+ on the 1st and 3rd postoperative day were significantly lower than preoperative level (P<0.05). In the trial group, percentages of CD3+, CD4+, and CD8+ on the 1st and 3rd postoperative day were significantly lower than preoperative levels(P<0.05), while percentages of CD3+, CD4+, and CD8+ on the 7th postoperative day were not statistically different from preoperative levels (P>0.05); CD4+ /CD8+ ratio on the 1st postoperative day was significantly lower than preoperative level (P<0.05), while CD4+ /CD8+ ratios on the 3rd and 7th postoperative day were not statistically different from preoperative level (P>0.05). There was no statistical difference in preoperative T lymphocyte subsets between the trial group and the control group (P>0.05). The percentages of CD4+ and CD4+/CD8+ ratio in the trial group were significantly higher than those of the control group on the 1st postoperative day (P<0.05), while the percentages of CD3+ and CD4+ and CD4+ /CD8+ratio in the trial group were significantly higher than those of the control group on the 3rd and 7th postoperative day(P<0.05). Conclusion Use of rhGH can significantly increase T lymphocyte subsets expression, enhance body cellular immunity, and improve postoperative recovery of patients with rheumatic valvular heart disease during the perioperative period of heart valve replacement.

    Release date:2016-08-30 05:46 Export PDF Favorites Scan
  • CHANGES IN PERIPHERAL BLOOD T LYMPHOCYTE SUBSETS OF RABBITS IN EARLY STAGE AFTER TRANSPLANTATION OF TISSUE ENGINEERED BONE CONSTITUTED BY BIOLOGICALLY-DERIVED SCAFFOLD

    Objective To observe the changes in the peripheral blood T lymphocyte subsets and the histomorphology of the transplanted tissues in the rabbits in the early stage after transplantation of the tissue engineered boneconstituted by the biologically-derived scaffold and to confirm the feasibility of the biologicallyderived materials as a scaffold in the bone tissue engineering. Methods Forty-eight healthy New Zealand rabbits (weight, 2.0-2.5 kg) with a 1-cm defect were equally and randomly divided into 4 groups: Groups A-D. The partial demineralized freeze-dried bone (PDFDB), the tissue engineered bone constructed by the osteoblasts derived from the lactant rabbit periosteum as a seeding cell, the xenogeneic cancellous bone undergoing the antigen self-digestion, partial demineralization and freeze-driedprocess as a scaffold, and the fresh xenogeneic allografting bone were respectively transplanted into the segmental defects of the rabbit radii in Groups A-D.To examine the effects of the 4 different materials, the flow cytometry was used to observe the changes in the T lymphocyte subsets in the rabbit peripheral blood at 1, 2, and 4 weeks after the operations and to examine the osteogenesis achieved by the 4 materials, the histological observations were also performed at 2, 4, 8, and 12 weeks after the operations. Results Two weeks after the tissue engineered bone transplantation in Group B, the osteoblasts and chondroblasts were found in the apertures of the scaffold, the new bone formation could be observed, the osteoclasts could be seen in the peripheral zone, and some of the netlike frameworks were destroyed and absorbed. Four weeks after the operation, the histological observation revealed that the osteocartilagionous callus turned into a woven bone. The peripheral blood T lymphocyte subsets of CD4+ and CD8+ were significantly greater in number 1-2 weeks after the operations and in Groups A and B than before the operations and in the other groups (.Plt;0.05);4 weeks after the operations the T lymphocyte subset of CD4+ was only slightly greater in number than before the operations, but with no statistically significant difference (Pgt;0.05). In Group C, the increase of the T lymphocyte subsets of CD4+ and CD8+ was not significant after the operation (Pgt;0.05). The T lymphocyte subsets of CD4+ and CD8+ were significantly greater in number 1, 2 and 4 weeks after the operations and in Group D than before the operation and in the other groups (Plt;0.05). Conclusion The tissue engineered bone constructed by the partial demineralized freezedried bone as a scaffold does not cause a serious immunologic rejection in the early stage after the transplantation and does not affect its good ability to repair the bone defect. The biologicallyderived bone canbe used as a scaffold in the bone tissue engineering.

    Release date:2016-09-01 09:22 Export PDF Favorites Scan
  • STUDY ON THE CHANGES AND INTERRELATIONSHIP OF SERUM IL-12 AND T LYMPHOCYTE SUBSET IN PATIENTS WITH PRIMARY HEPATIC CARCINOMA

    Objective To explore the changes and interrelationship of serum interleukin-12 (IL-12) and T lymphocyte subset in patients with primary hepatic carcinoma (PHC). Methods Serum IL-12 level was determined by ELISA in 36 patients with PHC. The peripheral blood T lymphocyte subset was assessed with flow cytometry. The distribution and changes of T lymphocyte subset in the tumor tissue were detected by immunohistochemistry analysis. Results The numbers of the CD+4 T cell were reduced and of the CD+8 T cell increased either in peripheral blood or tumor tissue, and showed the trend of the ratio (T4/T8) declined progressively with the aggravation of the state with PHC. IL-12 and T4/T8 had significant interrelationship.Conclusion IL-12 is an important antitumor factor of the patients with PHC. T lymphocyte subset plays a great role in the process of antitumor.

    Release date:2016-09-08 02:01 Export PDF Favorites Scan
  • 单核细胞 HLA-DR 和 T 淋巴细胞亚群预测严重创伤继发感染的临床研究

    目的 探讨连续监测单核细胞人类白细胞抗原 DR(HLA-DR)表达率变化及外周血 T 淋巴细胞亚群比例变化对严重创伤继发感染的预测价值。 方法 纳入 2014 年 6 月至 2016 年 6 月南京大学医学院附属鼓楼医院重症医学科收治的 26 例严重创伤患者,用流式细胞学方法检测其入院当天及第 3、5、7 d 外周血 HLA-DR 表达率及 T 淋巴细胞亚群比例,根据患者 28 d 内感染情况分为非感染组、局部感染组和全身感染组,分析 HLA-DR 表达率变化规律和 T 淋巴细胞亚群比例变化规律与感染的关系。 结果 26 例严重创伤患者中发生局部感染 10 例,全身感染12 例,感染率达 84.6%。与非感染组及局部感染组比较,全身感染组第 7 d 的 HLA-DR 表达率显著降低。与非感染组比较,局部感染组和全身感染组第 7 d 的 CD4+/CD8+ 比例显著降低。 结论 外周血单核细胞表面 HLA-DR 表达率及外周血 T 淋巴细胞亚群比例的连续监测在预测严重创伤患者继发感染、判断预后和预防治疗感染中具重要价值。

    Release date:2017-07-24 01:54 Export PDF Favorites Scan
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