This study aims to investigate the effect of lung ischemia reperfusion injury (LIRI) on expression of transient receptor potential vanilloid 1 (TRPV1) in the lung and brainstem of rats. Sixteen adult male Sprague Dawley rats weighing 250-320 g were randomly divided into Sham group and ischemia reperfusion group (IR group). Before ischemia, 0.5 hour and 4 hours after the reperfusion, respectively, arterial partial pressure of oxygen (PaO2) and arterial-alveolar oxygen pressure gradient (A-aDO2) were recorded and calculated, respectively. Left lung tissues and the brainstems were obtained at the end of the experiment. Lung tissue malondialdehyde (MDA), myeloperoxidase (MPO) activities, calcitonin gene related peptide (CGRP) and substance P (SP) levels were assessed. The mRNA and protein expressions of TRPV1 in the lung and brainstem were measured by qRT-PCR and Western blot. Compared with in the Sham group, rats in the IR group had a poorer blood gas exchange (P<0.05) and the MPO activity and MDA level of lung tissues in the IR group were significantly higher than those in the Sham group (P<0.05). CGRP level in the IR group increased remarkably (P<0.05), while SP level did not differ statistically between the two groups (P>0.05). The mRNA and protein expressions of TRPV1 in the lung tissue were upregulated in the IR group (P<0.05), but there were no differences of those in the brainstem between the two groups (P>0.05). The results suggest that LIRI could upregulate the expressions of TRPV1 and evoke CGRP release in the lung.
ObjectivesTo explore the effect of preoperative and early postoperative oral carbohydrate on the prognosis of patients undergoing colorectal cancer surgery.MethodsA total of 184 patients who underwent laparoscopic colorectal cancer surgery at the First Affiliated Hospital of Chongqing Medical University from March 2019 to July 2019 were selected. They were randomly divided into two groups: the drinking group (n=93) and the non-drinking group (n=91) using a random number table method. The drinking group drank 5 mL/kg carbohydrate clear drink 2 hours before surgery and began to take carbohydrate orally 4 hours after surgery. The non-drinking group was not allowed to drink for 8 hours before surgery and began to drink after exhaustion. The time to first flatus, first defecation in the two groups were observed. The incidence of complications (including aspiration, postoperative intestinal obstruction, anastomotic leakage, incision infection, postoperative nausea and vomiting, and pulmonary infection), postoperative length of stay and total cost of stay in the two groups were observed.ResultsCompared with the non-drinking group, the time to first flatus (Z=−8.009, P<0.001), the time to first defecation in the drinking group was significantly shorter (Z=−6.263, P<0.001), the incidence of postoperative complications was significantly lower (χ2=12.603, P<0.001), the length of postoperative hospitalization was shorter (t=−5.891, P<0.001). There was no statistical difference in total hospital expenses between the two groups (t=−1.860, P=0.065).ConclusionsOral administration of carbohydrate before and early after colorectal cancer surgery is safe and feasible, which can significantly reduce the overall complications, shorten the length of postoperative hospital stay, and promote the rapid recovery of patients.