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find Keyword "杂交" 86 results
  • EXPRESSION OF mRNA OF CD15 AND nm23H1 IN HEPATOCELLULAR CARCINOMA AND THEIR SIGNIFICANCE

    Objective To investigate the association of the expression of CD15 mRNA with the invasion and prognosis of hepatocellular carcinoma (HCC) and the expression of nm23H1 mRNA. Methods In situ hybridization and immunohistochemistry methods were used to detect the expression of CD15 mRNA and protein nm23H1 mRNA in HCC.Results In 99 cases of HCC, the positive rate of CD15 mRNA,its protein and nm23H1 mRNA were 38.4%, 36.4% and 76.8%, respectively. The expression of CD15 mRNA was consistent with its protein and negatively correlated with the expression of nm23H1 mRNA. The expression of CD15 mRNA and its protein, nm23H1 mRNA were associated with the invasiveness and metastasis of HCC and the prognosis of HCC patients. Conclusion The detection of CD15 expression could be a new pathological biology index to judge the metastasis and prognosis of HCC.

    Release date:2016-08-28 05:30 Export PDF Favorites Scan
  • Clinical research of complications and treatment of complex Stanford type B aortic dissection of hybrid surgical technique

    Objective To discusses the feasibilities of the hybrid surgical treatment of Stanford type B aortic dissection. Methods From August 2011 to August 2015 a total of 14 cases of complex Stanford type B aortic dissection patients had been completed hybrid surgery. Among them 11 cases of men and 3 cases of women, aged 22 to 62, an average of 44±7.2 years old. Twelve cases with dissecting aneurysm involving the aortic arch and its three vascular branch. There were 2 cases of patients after TEVER, occurred new dissection or pseudoaneurysms, and had hybrid surgery by traditional thoracotomy; 3 cases involving carotid artery were received neck-neck hybrid surgery, and 7 cases involving left subclavian artery were received neck-lock hybrid surgery. Two cases of dissecting aneurysm involving the iliac artery to thrombosis that result in lower limb ischemia, then femoral to femoral artery hybrid surgery were performed. Results All the patients were successfully completed the operation of covered stent implantation and hybrid surgery. Intraoperative angiography showed that the position of the stent was accurate, the interlayer isolation was successful, there was no obvious leakage and displacement of the stent, the true lumen blood flow of the aortic dissection was returned to normal, and bypass blood and target blood vessels were unobstructed. Fourteen patients were followed-up for a period of 3 to 36 months, with an average of (24.0±8.2) months. In 1 month after operation, pleural effusion occurred in 1 case, there was 1 case of cerebral stroke in two days after surgery, incision hematoma occurred in 1 case in 10 days after surgery, and the other patients had no postoperative death and severe complications. All 14 patients were followed-up and returned to normal life. Conclusion The hybrid operations can increase the success rate of TEVAR in complex Stanford type B aortic dissection patients, and early and mid-term results are satisfactory.

    Release date:2017-04-18 03:08 Export PDF Favorites Scan
  • 改良型杂交手术治疗DeBakeyⅠ型主动脉夹层一例

    Release date:2016-10-02 04:56 Export PDF Favorites Scan
  • THE FUNCTION OF NITRIC OXIDE IN THE NECROSIS OF AVULSED SKIN FLAP IN DOMESTIC PIG

    To investigate the function of nitric oxide (NO) and nitric oxide synthetase (NOS) inhibitor, N-nitro-L-arginine methyl ester (L-NAME), the skin avulsion model was made in the lower extremity of pig. The methods of measurement of size of the survived flap, weighing, immunocytochemistry and hybridization in situ were employed, so that the survival surface area of flaps, tissue wet/dry weight ratio, NO content in the serum, gene expression of NO and NOS content in the flap tissue were determined, respectively. The results showed that the early gene expression of NOS was increased as well as the NO content and tissue wet/dry weight ratio (P lt; 0.01). After L-NAME was applied introvenously, the NO content and tissue wet/dry weight ratio were decreased (P lt; 0.01), and the survival surface area of flaps was enlarged (P lt; 0.01). It could be concluded that the NO might play a role in the development of the pathological changes as early congestion, edema and secondary necrosis in the avulsed skin flaps. The early application of L-NAME could do some good to the avulsed skin flap and protect it from further necrosis owing to the presence of NO.

    Release date:2016-09-01 11:08 Export PDF Favorites Scan
  • Hybrid Procedures for Thoracoabdominal Aortic Aneurysm

    Release date:2016-09-08 10:45 Export PDF Favorites Scan
  • 食管异物及合并主动脉食管瘘的诊断与治疗

    目的探讨食管异物所致主动脉食管瘘的诊断及治疗方法。 方法回顾性分析2004年1月至2012年12月南昌大学第二附属医院32例异物性食管损伤患者的诊断和治疗经验。其中男21例、女11例,年龄18~78岁。采用多层螺旋CT(MSCT)及其影像后处理对食管异物病理变化进行准确分级;对主动脉食管瘘的患者采用包括杂交技术(覆膜支架腔内隔绝术+胸腔镜纵隔引流术)为主的治疗方案,总结MSCT和杂交技术在异物性主动脉食管瘘诊疗中的应用价值。 结果平扫影像诊断与临床诊断符合率为75%(24/32),影像后处理诊断与临床诊断符合率为100%(17/17)。MSCT分级所有Ⅰ级、Ⅱ级和Ⅲ级患者均治愈出院,4例Ⅳ级患者采用杂交技术治疗;7例Ⅳ级主动脉食管瘘患者中有5例痊愈出院,2例死亡,因开胸行主动脉修补术中死亡1例,开胸行主动脉置换术后24 h内死亡1例。30例痊愈出院患者随访6个月,除1例Ⅳ级患者有轻微背痛以外,其他患者无不适。 结论MSCT对食管异物性损伤的准确分级和治疗方式选择均有重要的价值,杂交技术治疗异物性主动脉食管瘘具有重要的临床意义。

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  • Treatment choices of chronic thoracoabdominal aortic dissection aneurysm

    The treatment of chronic thoracoabdominal aortic dissection aneurysm remains a major challenge in aortic surgery. Open surgery is the mainstream treatment at present. New devices for endovascular treatment of chronic thoracoabdominal aortic dissection are gradually applied in clinical practice. The hybrid procedure is a combination of open and endovascular procedures. The appropriate treatment should be selected according to the patient's age, anatomy, genetic aortic disease, and comorbidities.

    Release date:2024-06-20 05:33 Export PDF Favorites Scan
  • Expression of connective tissue growth factor in injured model of retinal pigment epithelial cells and the promoting effect on migration

    ObjectiveTo observe the expression of connective tissue growth factor (CTGF) in injured model of retinal pigment epithelial (RPE) cells and the promoting effect of CTGF on migration of RPE cells.MethodsCultured monolayer-confluent human RPE cells were scraped with a trephine and a cotton stick, and set up the injured model of RPE cells with round scraped area. Immunohistochemistry and in situ hybridization(ISH) were used to detect the expression of CTGF protein and mRNA in injured RPE cells at distinct time points after injury. The number of RPE cells migrated to injured area was measured and the effect of CTGF on migration of RPE cells and the effect of dexamethasone (DEX) on the promoting process of CTGF were observed.ResultsThe results of immunohstochemistry and ISH indicated the weak positive expression of CTGF in RPE cells at the edge of scrape 6 hours after injury, and the positive expression increased gradually as time goes by after the injury. Strong positive expression of CTGF in RPE cells at the edge of scrape was found 24 and 48 hours after injury. Rebuilt human CTGF stimulated migration of RPE cells in a dose-depended manner, and DEX significantly inhabited the migration.ConclusionCTGF involves in the procedure of repair of injury of RPE cells, which may play an important role in the pathogenesis of intraocular proliferative diseases such as proliferative vitreoretinaopathy.(Chin J Ocul Fundus Dis, 2005,21:306-309) 

    Release date:2016-09-02 05:52 Export PDF Favorites Scan
  • Open Surgical Repair Combined with Endovascular Repair for Arterial Disease

    Release date:2016-09-08 10:45 Export PDF Favorites Scan
  • 分期杂交手术治疗胸腹主动脉夹层

    目的总结腹主动脉去分支化手术联合主动脉腔内隔绝术治疗胸腹主动脉夹层的经验。方法自 2018 年 1 月至 2019 年 6 月,南京鼓楼医院采用腹主动脉去分支化手术联合主动脉腔内隔绝术治疗胸腹主动脉夹层 12 例,男 10 例、女 2 例,年龄 36~63(50±9)岁。结果随访 6~24 个月,全组无死亡,1 例术后左下肢单瘫,1 例出现肾功能不全。随访期间 1 例患者术后 1 个月出现主动脉支架近端逆撕,行全主动脉弓置换术后痊愈。2 例患者出现腹腔干动脉近端内漏,予以弹簧圈栓塞后痊愈,其余患者 CT 血管造影(CTA)检查未见吻合口造影剂渗漏及人工血管扭曲、闭塞。结论腹主动脉去分支化手术联合主动脉腔内隔绝术治疗胸腹主动脉夹层可取得良好近期疗效,中远期疗效仍有待进一步观察随访。

    Release date:2020-09-22 02:51 Export PDF Favorites Scan
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