Objective To explore the effects of bile from patients with cholecystolithiasis on the growth of human gallbladder carcinoma cells GBC-SD and the potential correlation between cholecystolithiasis and gallbladder carcinoma. Methods Cholecystolithiasis bile (CB) and normal bile (NB) specimens were used for this study. The proliferative effects of bile were measured by methabenzthiazuron (MTT) assay and cell cycle and apoptosis were analyzed by flow cytometry. Results CB can significantly promote the proliferation of GBC-SD cells, GBC-SD proliferative index increased significantly after treated with 1% CB for 48 h （P＜0.05）.The Sphase fraction of CB 〔（49.26±8.07）%〕 increased remarkably (P＜0.05) compared with that of NB 〔（25.54±6.57）%〕, and the CB percentage of G0/G1 phase 〔（40.59±9.12）%〕 decreased remarkably (P＜0.05) compared with NB 〔(60.64±13.42）〕%. Conclusion CB can promote the proliferation of human gallbladder carcinoma GBC-SD cells.
目的:探讨腹腔镜胆囊切除术(LC)与内镜十二指肠乳头括约肌切开术(EST)联合应用治疗胆囊结石合并胆总管结石的临床效果。方法:回顾性分析我院开展的LC联合EST治疗胆囊结石合并胆总管结石76例,其中56例先行EST后行LC,20例先行LC后行ERCP/EST。结果:本组全部治愈,先行EST组56例,3例并发胰腺炎,3例出血,2例再发胆总管结石,先行LC组20例行EST11例,6例取石后未做括约肌切开,3例结石自行掉入肠道,1例出现胆道感染,1例胰腺炎,无出血及穿孔。结论:内镜治疗胆囊结石继发胆总管结石具有创伤小、效果好、并发症少、恢复快的的特点;先作EST可解除胆道梗阻、减轻炎症,并为LC创造条件,选择性先行LC后可减轻创伤,甚至不必做EST。
ObjectiveTo summarize the experience of the transabdominal reverse guide technique of endoscopic nasobiliary drainage during the course of laparoscopic common bile duct exploration with endoscopic nasobiliary drainage (LENBD). MethodsFrom May 2008 to March 2015, there were 237 cases undergoing LENBD, involving choledochotomy, electrohydralic lithothipsy, incision on the stenosis of papillary, bile duct drainage through the endoscopic nasobiliary drainage, and primary closure of duct incision. ResultsEndoscopic nasobiliary drainage:the procedure were successful in 215 cases (90.7%) of nasobiliary drainage out of 237 cases. Nasal bile duct intubation failed in 6 cases (2.5%), wherein the transfered for ureteral catheter drainage of bile duct in 5 cases, bile duct drainage was given up in 1 cases. Nasobiliary discount caused no liquid outflow in 9 cases (3.8%). Nasal bile duct early slipped in 7 cases (3.0%). Primary closure of bile duct incision:the procedure were successful in 229 cases (96.6%) of primary closure out of 237 cases. Primary closure failed in 8 cases (3.4%) that the transfered for ureteral catheter drainage of bile duct in 5 cases (2.1%) and the bile leakage occurred in 3 cases (1.3%) with primary closure of duct incision and cured by patent drainage. Six cases (2.5%) were a slight pancreatitis after operation. Head of the nose bile duct was wrong to sew in 1 case (0.4%). No case residual stones in the biliary duct of 237 cases. No other serious complications and no case died postoperatively. ConclusionIf patients are suitable, transabdominal reverse guide technique in laparoscopic common bile duct exploration of endoscopic nasobiliary drainage is safe and effective in the hands of skilled endoscopists.